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Biochemical Society Transactions (2006) 34, (156–159) (Printed in Great Britain)

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A multitude of CRP/FNR-like transcription proteins in Bradyrhizobium japonicum

S. Mesa¹, H. Hennecke and H.-M. Fischer

Institute of Microbiology, Eidgenössische Technische Hochschule, CH-8093 Zürich, Switzerland

Key words: Bradyrhizobium japonicum, cAMP receptor protein/fumarate and nitrate reductase regulatory protein-like transcription factor (CRP/FNR-like transcription factor), denitrification, gene regulation, nitrogen fixation, symbiosis.

Abbreviations used: CRP, cAMP receptor protein; FNR, fumarate and nitrate reductase regulatory protein; NnrR, nitrite and nitric oxide reductase regulator.

¹To whom correspondence should be addressed (email mesam@micro.biol.ethz.ch).

Abstract

In Bradyrhizobium japonicum, the nitrogen-fixing soya bean endosymbiont and facultative denitrifier, three CRP (cAMP receptor protein)/FNR (fumarate and nitrate reductase regulatory protein)-type transcription factors [FixK₁, FixK₂ and NnrR (nitrite and nitric oxide reductase regulator)] have been studied previously in the context of the regulation of nitrogen fixation and denitrification. The gene expression of both fixK₁ and nnrR depends on FixK₂, which acts as a key distributor of the ‘low-oxygen’ signal perceived by the two-component regulatory system FixLJ. While the targets for FixK₁ are not known, NnrR transduces the nitrogen oxide signal to the level of denitrification gene expression. Besides these three regulators, the complete genome sequence of this organism has revealed the existence of 13 additional CRP/FNR-type proteins whose functions have not yet been studied. Based on sequence similarity and phylogenetic analysis, we discuss in this paper the peculiarities of these additional factors.

Received 23 September 2005

© 2006 Biochemical Society