Pluripotent embryonic stem cells can give rise to neuroectodermal derivatives in culture. This potential could be harnessed to generate neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery. However, current methods of neural differentiation are empirical and relatively innefficient. Here, we review these methodologies and present new tools for quantification, analysis and manipulation of embryonic stem cell neural determination.
- in vitro neurogenesis
- neural progenitor
Signalling the Future, a Biochemical Society-sponsored meeting held at University of Liverpool, Liverpool, 4–6 September 2002
Abbreviations used: BMP, bone morphogenetic protein; EB, embryoid body; ES, embryonic stem; FACS, fluorescence-activated cell sorting; FGF, fibroblast growth factor; GFP, green fluorescent protein; LIF, leukaemia inhibitory factor; Shh, Sonic hedgehog.
- Copyright 2003 Biochemical Society