Lymphocytes from the blood home to secondary lymphoid tissues through a process of tethering, rolling, firm adhesion and transmigration. Tethering and rolling of lymphocytes is mediated by the interaction of L-selectin on lymphocytes with sulphated ligands expressed by the specialized endothelial cells of high endothelial venules (HEVs). The sulphate-dependent monoclonal antibody MECA79 stains HEVs in peripheral lymph nodes and recognizes the complex of HEV ligands for L-selectin termed peripheral node addressin. High endothelial cell GlcNAc-6-sulphotransferase/L-selectin ligand sulphotransferase is a HEV-expressed sulphotransferase that contributes to the formation of the MECA79 epitope and L-selectin ligands on lymph node HEVs. MECA79-reactive vessels are also common at sites of chronic inflammation, suggesting mechanistic parallels between lymphocyte homing and inflammatory trafficking.
- lymphocyte recirculation
678th Meeting of the Biochemical Society, held at Imperial College, London, 16–18 December 2002
Abbreviations used: FucT, fucosyltransferase; GlyCAM-1, glycosylation-dependent cell adhesion molecule-1; HEC, high endothelial cell; HEC-GlcNAc6ST, high endothelial cell GlcNAc-6-sulphotransferase; HEV, high endothelial venule; LSST, L-selectin ligand sulphotransferase; PNAd, peripheral node addressin; PSGL-1, P-selectin glycoprotein ligand; sLex, sialyl Lewis X.
- Copyright 2003 Biochemical Society