Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.
- cytochrome P450 reductase
- electron transfer
Enzyme Mechanism – A Structural Perspective, a Biochemical Society Focused Meeting held at St. Andrews University, 12–14 January 2003
Abbreviations used: CPR, cytochrome P450 reductase; hq, hydroquinone; ox, oxidized; sq, semiquinone.
- © 2003 Biochemical Society