Enzyme Mechanism – A Structural Perspective

Electron transfer in human cytochrome P450 reductase

A. Gutierrez, A. Grunau, M. Paine, A.W. Munro, C.R. Wolf, G.C.K. Roberts, N.S. Scrutton


Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.

  • cytochrome P450 reductase
  • electron transfer
  • flavoprotein
  • kinetics


  • Enzyme Mechanism – A Structural Perspective, a Biochemical Society Focused Meeting held at St. Andrews University, 12–14 January 2003

  • Abbreviations used: CPR, cytochrome P450 reductase; hq, hydroquinone; ox, oxidized; sq, semiquinone.