c-Type cytochromes are a widespread class of proteins that play a vital role in the energy-conserving metabolism of prokaryotic and eukaryotic organisms. The key event in cytochrome c biogenesis is the covalent attachment of the haem cofactor to two (or rarely one) cysteine residues arranged in a haem c-binding motif such as CX2–4CH, CXXCK or X3CH. This reaction is catalysed by the membrane-bound enzyme CCHL (cytochrome c haem lyase). Different CCHLs have been described and some of them are dedicated to distinct haem c-binding motifs of cytochromes that are encoded in the vicinity of the respective CCHL gene. Various bacterial genomes contain multiple copies of CCHL-encoding genes, suggesting the presence of non-conventional or even as yet unrecognized haem c-binding motifs. This assumption is exemplified in the present study using the proteobacterium Wolinella succinogenes as a model organism whose genome encodes three CCHL isoenzymes. The discovery of a novel conserved multihaem cytochrome c (MccA) is described.
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February 2006
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Conference Article|
January 20 2006
Multiple haem lyase genes indicate substrate specificity in cytochrome c biogenesis
S. Hartshorne;
S. Hartshorne
* School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
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D.J. Richardson;
D.J. Richardson
* School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
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J. Simon
J. Simon
1
* School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
† Institut für Molekulare Biowissenschaften, Johann Wolfgang Goethe-Universität, Marie-Curie-Strasse 9, D-60439 Frankfurt am Main, Germany
1 To whom correspondence should be addressed, at Institut für Molekulare Biowissenschaften, Johann Wolfgang Goethe-Universität (email j.simon@em.uni-frankfurt.de).
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Publisher: Portland Press Ltd
Received:
September 22 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (1): 146–149.
Article history
Received:
September 22 2005
Citation
S. Hartshorne, D.J. Richardson, J. Simon; Multiple haem lyase genes indicate substrate specificity in cytochrome c biogenesis. Biochem Soc Trans 1 February 2006; 34 (1): 146–149. doi: https://doi.org/10.1042/BST0340146
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