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Recent Advances in Membrane Biochemistry

Surfactant-free purification of membrane proteins with intact native membrane environment

Mohammed Jamshad, Yu-Pin Lin, Timothy J. Knowles, Rosemary A. Parslow, Craig Harris, Mark Wheatley, David R. Poyner, Roslyn M. Bill, Owen R.T. Thomas, Michael Overduin, Tim R. Dafforn
Biochemical Society Transactions May 20, 2011, 39 (3) 813-818; DOI: 10.1042/BST0390813
Mohammed Jamshad
School of Biosciences, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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Yu-Pin Lin
School of Biosciences, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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Timothy J. Knowles
School of Cancer Studies, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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Rosemary A. Parslow
School of Biosciences, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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Craig Harris
School of Biosciences, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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Mark Wheatley
School of Biosciences, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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David R. Poyner
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Roslyn M. Bill
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Owen R.T. Thomas
school of Chemical Engineering, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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Michael Overduin
School of Cancer Studies, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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Tim R. Dafforn
School of Biosciences, University of Birmingham, Birmingham, Edgbaston B15 2TT, U.K.
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  • For correspondence: t.r.dafforn@bham.ac.uk
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Abstract

In order to study the structure and function of a protein, it is generally required that the protein in question is purified away from all others. For soluble proteins, this process is greatly aided by the lack of any restriction on the free and independent diffusion of individual protein particles in three dimensions. This is not the case for membrane proteins, as the membrane itself forms a continuum that joins the proteins within the membrane with one another. It is therefore essential that the membrane is disrupted in order to allow separation and hence purification of membrane proteins. In the present review, we examine recent advances in the methods employed to separate membrane proteins before purification. These approaches move away from solubilization methods based on the use of small surfactants, which have been shown to suffer from significant practical problems. Instead, the present review focuses on methods that stem from the field of nanotechnology and use a range of reagents that fragment the membrane into nanometre-scale particles containing the protein complete with the local membrane environment. In particular, we examine a method employing the amphipathic polymer poly(styrene-co-maleic acid), which is able to reversibly encapsulate the membrane protein in a 10 nm disc-like structure ideally suited to purification and further biochemical study.

  • amphipol
  • bicelle
  • membrane protein
  • nanoparticle
  • solubilization
  • surfactant

Footnotes

  • Recent Advances in Membrane Biochemistry: Biochemical Society Annual Symposium No. 78 held at Robinson College, Cambridge, U.K., 5–7 January 2011. Organized and Edited by J. Malcolm East (Southampton, U.K.) and Frank Michelangeli (Birmingham, U.K.).

Abbreviations: AUC, analytical ultracentrifugation; DHPC, 1,2-dihexanoyl-sn-glycero-3-phosphocholine; GPCR, G-protein-coupled receptor; MSP, membrane scaffold protein; SMA, poly(styrene-co-maleic acid); SMALP, SMA lipid particle

  • © The Authors Journal compilation © 2011 Biochemical Society
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June 2011

Volume: 39 Issue: 3

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Surfactant-free purification of membrane proteins with intact native membrane environment
Mohammed Jamshad, Yu-Pin Lin, Timothy J. Knowles, Rosemary A. Parslow, Craig Harris, Mark Wheatley, David R. Poyner, Roslyn M. Bill, Owen R.T. Thomas, Michael Overduin, Tim R. Dafforn
Biochemical Society Transactions Jun 2011, 39 (3) 813-818; DOI: 10.1042/BST0390813
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Surfactant-free purification of membrane proteins with intact native membrane environment
Mohammed Jamshad, Yu-Pin Lin, Timothy J. Knowles, Rosemary A. Parslow, Craig Harris, Mark Wheatley, David R. Poyner, Roslyn M. Bill, Owen R.T. Thomas, Michael Overduin, Tim R. Dafforn
Biochemical Society Transactions Jun 2011, 39 (3) 813-818; DOI: 10.1042/BST0390813

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Keywords

amphipol
bicelle
membrane protein
nanoparticle
solubilization
surfactant

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