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Molecular Biology of Archaea 3

Archaeal transcription: making up for lost time

Magdalena N. Wojtas, Nicola G.A. Abrescia
Biochemical Society Transactions Jan 29, 2013, 41 (1) 356-361; DOI: 10.1042/BST20120305
Magdalena N. Wojtas
Structural Biology Unit, CIC bioGUNE, CIBERehd, 48160 Derio, Spain
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Nicola G.A. Abrescia
Structural Biology Unit, CIC bioGUNE, CIBERehd, 48160 Derio, SpainIKERBASQUE, Basque Foundation for Science, 48011 Bilbao, Spain
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Abstract

In recent years, emerging structural information on the aRNAP (archaeal RNA polymerase) apparatus has shown its strong evolutionary relationship with the eukaryotic counterpart, RNA Pol (polymerase) II. A novel atomic model of SshRNAP (Sulfolobus shibatae RNAP) in complex with dsDNA (double-stranded DNA) constitutes a new piece of information helping the understanding of the mechanisms for DNA stabilization at the position downstream of the catalytic site during transcription. In Archaea, in contrast with Eukarya, downstream DNA stabilization is universally mediated by the jaw domain and, in some species, by the additional presence of the Rpo13 subunit. Biochemical and biophysical data, combined with X-ray structures of apo- and DNA-bound aRNAP, have demonstrated the capability of the Rpo13 C-terminus to bind in a sequence-independent manner to downstream DNA. In the present review, we discuss the recent findings on the aRNAP and focus on the mechanisms by which the RNAP stabilizes the bound DNA during transcription.

  • Archaea
  • RNA polymerase
  • Rpo13
  • transcription
  • X-ray structure

Footnotes

  • Molecular Biology of Archaea 3: An Independent Meeting held at the Max Planck Institute for Terrestrial Microbiology, Marburg, Germany, 2–4 July 2012. Organized and Edited by Sonja-Verena Albers (Max Planck Institute for Terrestrial Microbiology, Germany), Bettina Siebers (University of Duisberg-Essen, Germany) and Finn Werner (University College London, U.K.).

Abbreviations: aRNAP, archaeal RNA polymerase; dsDNA, double-stranded DNA; electron, microscopy; eRNAP, eukaryotic RNA polymerase; LACA, last archaeal common ancestor; OC, open complex; PfuRNAP, Pyrococcus furiosus RNA polymerase; Pol, polymerase; RNAP, RNA polymerase; SshRNAP, Sulfolobus shibatae RNAP; TBP, TATA-box-binding protein; TEC, transcription elongation complex; TF, transcription factor

  • © The Authors Journal compilation © 2013 Biochemical Society
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February 2013

Volume: 41 Issue: 1

Biochemical Society Transactions: 41 (1)
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Archaeal transcription: making up for lost time
Magdalena N. Wojtas, Nicola G.A. Abrescia
Biochemical Society Transactions Feb 2013, 41 (1) 356-361; DOI: 10.1042/BST20120305
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Archaeal transcription: making up for lost time
Magdalena N. Wojtas, Nicola G.A. Abrescia
Biochemical Society Transactions Feb 2013, 41 (1) 356-361; DOI: 10.1042/BST20120305

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  • Article
    • Abstract
    • Introduction
    • Desalting of SshRNAP crystals in droplets
    • Overall structure of the aRNAP–DNA binary complex
    • DNA stabilization in archaeal transcription
    • The RNAP subunits bolt-on model
    • Rpo13 subunit: an ancestral bound transcription factor-like protein?
    • Conclusions
    • Funding
    • Acknowledgments
    • Footnotes
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Keywords

Archaea
RNA polymerase
Rpo13
transcription
X-ray structure

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