RNA granules have been observed in different organisms, cell types and under different conditions, and their formation is crucial for the mRNA life cycle. However, very little is known about the molecular mechanisms governing their assembly and disassembly. The aggregation-prone LSCRs (low-sequence-complexity regions), and in particular, the polyQ/N-rich regions, have been extensively studied under pathological conditions due to their role in neurodegenerative diseases. In the present review, we discuss recent in vitro, in vivo and computational data that, globally, suggest a role for polyQ/N regions in RNA granule assembly.
- low-sequence-complexity region (LSCR)
- polyQ/(N) region
- RNA-binding protein
- RNA granule
RNA UK 2014: An Independent Meeting held at Low Wood Hotel, Windermere, U.K., 24–26 January 2014. Organized and Edited by Niki Gray, Gracjan Michlewski and Steve West (University of Edinburgh, U.K.).
Abbreviations: b-isox, biotinylated isoxazole; eIF, eukaryotic initiation factor; LSCR, low-sequence-complexity region; MEF, mouse embryonic fibroblast; miRISC, miRNA-induced silencing complex; P-body, processing body; RBD, RNA-binding domain; RBP, RNA-binding protein; TIA-1, T-cell intracellular antigen-1; TNRC6, trinucleotide repeat-containing protein 6; Tud, Tudor; WT, wild-type
- © The Authors Journal compilation © 2014 Biochemical Society