Cytokinesis separates the forming daughter cells. Higher plants have lost the ability to constrict the plasma membrane (PM) in the division plane. Instead, trans-Golgi network (TGN)-derived membrane vesicles are targeted to the centre of the division plane and generate, by homotypic fusion, the partitioning membrane named cell plate (CP). The CP expands in a centrifugal fashion until its margin fuses with the PM at the cortical division site. Mutant screens in Arabidopsis have identified a cytokinesis-specific syntaxin named KNOLLE and an interacting Sec1/Munc18 (SM) protein named KEULE both of which are required for vesicle fusion during cytokinesis. KNOLLE is only made during M-phase, targeted to the division plane and degraded in the vacuole at the end of cytokinesis. Here we address mechanisms of KNOLLE trafficking and interaction of KNOLLE with different soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) partners and with SM-protein KEULE, ensuring membrane fusion in cytokinesis.
- cell plate
- membrane fusion
- membrane traffic
- soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)
The Dynamic Cell: held at Robinson College, Cambridge University, Cambridge, U.K., 4–7 September 2014.
Abbreviations: ARF-GEF, adenosine ribosylation factor guanine-nucleotide exchange factor; BFA, brefeldin A; BIG, BFA-inhibited guanine-nucleotide exchange factor (GEF); CP, cell plate; EE, early endosome; ER, endoplasmic reticulum; GNL1, GNOM-LIKE1; MVB, multivesicular body; NSF, N-ethylmaleimide-sensitive factor; PEN1, PENETRATION1; PHP, phragmoplast microtubules; PM, plasma membrane; SM, Sec1/Munc18; SNARE, soluble NSF attachment protein (SNAP) receptor; TGN, trans-Golgi network; VAMP, vesicle-associated membrane protein
- © The Authors Journal compilation © 2015 Biochemical Society