Table 1 Phenotype of cell populations analysed by transcriptional profiling
Cell populationPhenotypeStageProfilingReference
Non-HECs
HECs
HSPCs
Runx1 + 23GFPCdh5+Ter119CD45CD41
Runx1 + 23GFP+Cdh5+Ter119CD45CD41
Runx1 + 23GFP+Cdh5+Ter119CD45CD41+
E8.5, E9.25, E10.5Microarrays and scqRT-PCR[49]
Non-HECs
HECs
HSCs
HPCs
Ly6a-GFPCD31+ckit
Ly6a-GFP+CD31+ckit
Ly6a-GFP+CD31+ckit+
Ly6a-GFPCD31+ckit+
E10.5Bulk RNA-Seq[70]
GFP+ ECs
GFP ECs
GFP+ HCCs
GFP HCCs
Ly6a-GFP+CD31+Cdh5+Esam+ckit
Ly6a-GFPCD31+Cdh5+Esam+ckit
Ly6a-GFP+CD31+Cdh5+Esam+ckit+
Ly6a-GFPCD31+Cdh5+Esam+ckit+
E10.5Bulk RNA-Seq[68]
Trunk HECs
Trunk rest
runx1-GFP+
runx1-GFP
26–28 hpfBulk RNA-Seq[67]
ECs
Pre-HSC I
Pre-HSC II
HSC (FL)
HSC (FL)
CD31+Cdh5+CD41CD43CD45Ter119
CD31+CD45CD41locKit+EPCRhi
CD31+CD45+CD41lo
LinSca1+Mac1loEPCR+
CD45+CD150+CD48EPCR+
E11.5
E11.5
E11.5
E12
E14
10-cell and scRNA-Seq[71]
HCCs
Non-HECs
HECs
EHT cells
Pre-HSC I
Pre-HSC II
HSPCs (YS)
ckit+
Cdh5+Gfi1-tomatoGfi1b-GFPckit
Cdh5+Gfi1-tomato+Gfi1b-GFPckit
Cdh5+Gfi1-tomato+Gfi1b-GFPckit+
Cdh5+ckit+CD45
Cdh5+ckit+CD45+
ckit+
E11.5
E10/E11


E11

E10/E11
scRNA-Seq[66]
ECs-HSPCs
Non-HECs
HECs
Pre-HSPC I
Pre-HSPC II
Pro-HSCs
Pre-HSC I
Pre-HSC II
Non-HECs
HECs
Pre-HSPC I
Pre-HSPC II
Cdh5+
Cdh5+CD44
Cdh5+CD44lockit
Cdh5+CD44lockit+
Cdh5+CD44hi
Cdh5+CD41+CD43CD45
Cdh5+CD41+CD43+CD45
Cdh5+CD41+CD43+CD45+
Cdh5+CD44
Cdh5+CD44lockit
Cdh5+CD44lockit+
Cdh5+CD44hi
E10.5
E10



E10


E9.5/10/11

E9.5/10
E11
scRNA-Seq
scqRT-PCR



scqRT-PCR


Bulk RNA-Seq
[69]

Abbreviations: EC, endothelial cell; EHT, endothelial-to-haematopoietic transition; FL, foetal liver; HEC, haemogenic endothelial cell; HCC, haematopoietic cluster cell; HPC, haematopoietic progenitor cell; hpf, hours post fertilisation; HSC, haematopoietic stem cell; HSPC, haematopoietic stem/progenitor cell; sc, single cell; YS, yolk sac.